Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2F7.07c
Gene ID 45/H11
Gene synonyms/obsolete
Gene product zinc finger protein; zf-PHD finger; involved in transcriptional regulation
Entry clone Cloned
ORF length (unspliced) 1824
ORF length (spliced)
Entry clone length 1824
No. of intron 0
Sequence status Finished
Sequence results 643A:addition
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC2F7.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGCAAAACCATGGAA
Rev primer name SPAC2F7.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATAGCTTAATAAGTTAGCC
Amino acid length 607
Molecular weight 68.7
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 110
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; nuclear dots; dots on spindle microtubules
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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