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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F7.08
Gene ID 46/A07
Gene synonyms/obsolete fio1
Gene product iron transport multicopper oxidase
Entry clone Cloned
ORF length (unspliced) 1869
ORF length (spliced)
Entry clone length 1869
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1F7.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATAAATTTTTCTCCTT
Rev primer name SPAC1F7.08.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTATTTCATCTTTTCCT
Amino acid length 622
Molecular weight 69.9
Isoelectric point (calc.) 4
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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