| Gene name |
SPAC2F3.15 |
| Gene ID |
46/B09 |
| Gene synonyms/obsolete |
|
| Gene product |
putative cell division
protein kinase; serine/threonine protein kinase |
| Entry clone |
Cloned; Mixture |
| ORF length (unspliced) |
1945 |
| ORF length (spliced) |
1782 |
| Entry clone length |
1945 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
Mixture |
| Comments |
Sequence quality is
low / Mixture of 2 clones, one of which is frameshifted from
somewhere. |
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC2F3.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCATACTCGAAGAGTAC |
| Rev primer name |
SPAC2F3.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCTTTTAGATTTTCGTTTT |
| Amino acid length |
593 |
| Molecular weight |
67.3 |
| Isoelectric point (calc.) |
10.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |