| Gene name |
SPBC24C6.02 |
| Gene ID |
46/B11 |
| Gene synonyms/obsolete |
|
| Gene product |
putative ATP dependent
RNA helicase DEAD/DEAH box helicase; ATP-dependent; RNA
helicase; helicase C-terminal domain; involved in 35S
transcript processing; involved in ribosome biogenesis and
assembly |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1950 |
| ORF length (spliced) |
1821 |
| Entry clone length |
1950 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC24C6.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTTTCAGAGTATTAA |
| Rev primer name |
SPBC24C6.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACATACTAAATCCAAAGGAT |
| Amino acid length |
606 |
| Molecular weight |
68.7 |
| Isoelectric point (calc.) |
10.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
531/493 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |