| Gene name |
SPAC23E2.03c |
| Gene ID |
46/C06 |
| Gene synonyms/obsolete |
ste7 |
| Gene product |
required for mating
and meiosis; involved in conjugation (required); involved in
meiosis (required); no apparent orthologs |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1710 |
| ORF length (spliced) |
|
| Entry clone length |
1710 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC23E2.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTTTGGAAATAATCCT |
| Rev primer name |
SPAC23E2.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGAGACTGTAAGACTTCA |
| Amino acid length |
569 |
| Molecular weight |
61.1 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB?; nuclear dots;
periphery at site of septum formation; cytosol; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |