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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC18G6.10
Gene ID 46/D11
Gene synonyms/obsolete
Gene product hypothetical protein
Entry clone Cloned
ORF length (unspliced) 2067
ORF length (spliced)
Entry clone length 2067
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC18G6.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGACAATTGGGAGGACCC
Rev primer name SPAC18G6.10.Rv
Rev primer SEQ AGAAAGCTGGGTATTCACGTAAAGGGGAAGTT
Amino acid length 688
Molecular weight 78.1
Isoelectric point (calc.) 8.3
Signal SEQ
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear envelope; periphery
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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