| Gene name |
SPBC17D11.02c |
| Gene ID |
46/E04 |
| Gene synonyms/obsolete |
|
| Gene product |
C3H4 type zinc finger
protein; zf-C3HC4 type (RING finger); ubiquitin ligase (E3)
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
2077 |
| ORF length (spliced) |
2034 |
| Entry clone length |
2077 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC17D11.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGTTTATACTATATGT |
| Rev primer name |
SPBC17D11.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAATTTTCATCCACAGTT |
| Amino acid length |
677 |
| Molecular weight |
75.6 |
| Isoelectric point (calc.) |
6.4 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
6 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYVLASLVL |
| Localization (YFP) |
large bright dots;
small cytoplasmic dots at periphery |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |