Gene name |
SPBC1718.04 |
Gene ID |
46/E05 |
Gene synonyms/obsolete |
|
Gene product |
phosphate
acyltransferase (SMART); involved in phospholipid
biosynthesis; SCT1 is a suppressor of a choline transport
mutant; similar to protein mutated in Barth syndrome |
Entry clone |
Cloned |
ORF length (unspliced) |
2080 |
ORF length (spliced) |
2028 |
Entry clone length |
2080 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC1718.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAACATACCTTTATTTA |
Rev primer name |
SPBC1718.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTCACTTAATTCTTCGTCA |
Amino acid length |
675 |
Molecular weight |
76.6 |
Isoelectric point (calc.) |
9.4 |
Signal SEQ |
|
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |