| Gene name |
SPAC17G6.18 |
| Gene ID |
46/E07 |
| Gene synonyms/obsolete |
SPAC1142.01 |
| Gene product |
conserved eukaryotic
protein; predicted coiled-coil region; DUF654; similar to
mouse nulp1 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2098 |
| ORF length (spliced) |
2004 |
| Entry clone length |
2098 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC17G6.18.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAATATTTTCTGTATGT |
| Rev primer name |
SPAC17G6.18.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCCCCTTGACCCATCTCT |
| Amino acid length |
667 |
| Molecular weight |
76.3 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
99 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSELLDTLNI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |