| Gene name |
SPAC6F12.12 |
| Gene ID |
46/F01 |
| Gene synonyms/obsolete |
par2; pbp2 |
| Gene product |
protein phosphatase
PP2A, B' regulatory subunit; involved in cytokinesis; involved
in cellular morphogenesis; involved in stress tolerance |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2129 |
| ORF length (spliced) |
1884 |
| Entry clone length |
2129 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC6F12.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAGGATTAAGGAGTAA |
| Rev primer name |
SPAC6F12.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCTTAAATAATCAGTGGGT |
| Amino acid length |
627 |
| Molecular weight |
72.4 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRSKFVKALSL/LEEVYLLFI |
| Localization (YFP) |
periphery at cell tip
and site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |