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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19G12.07c
Gene ID 46/F08
Gene synonyms/obsolete
Gene product involved in mRNA splicing (implicated); no apparent Sc ortholog; rrm RNA recognition motif; RNA-binding protein
Entry clone Cloned
ORF length (unspliced) 2208
ORF length (spliced) 1815
Entry clone length 2208
No. of intron 6
Sequence status Finished
Sequence results 667A:G / 1870T:C
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC19G12.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGCTGGACGCGGAAGC
Rev primer name SPAC19G12.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAGTTTTGGCATTAGGG
Amino acid length 604
Molecular weight 69.4
Isoelectric point (calc.) 9.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 218/217
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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