Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC365.10
Gene ID 46/G02
Gene synonyms/obsolete
Gene product actin-like protein; involved in intracellular protein transport; involved in protein-vacuolar targeting
Entry clone Cloned
ORF length (unspliced) 2221
ORF length (spliced) 2166
Entry clone length 2221
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC365.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAATATATGCTGTTAG
Rev primer name SPBC365.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAATTTATGTTTCCTAAA
Amino acid length 721
Molecular weight 82.3
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDYTFLKLGI
Localization (YFP) nucleus>>cytosol
Comments for localization cytoplasmic aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.