Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19G12.01c
Gene ID 46/H03
Gene synonyms/obsolete cut20; lid1; SPAPJ698.04c
Gene product cyclosome/apc subunit; Cut20/Apc4 anaphase-promoting complex (APC); cyclosome; WD repeat protein (putative); involved in metaphase-anaphase transition (regulation)
Entry clone Cloned
ORF length (unspliced) 2386
ORF length (spliced) 2160
Entry clone length 2386
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC19G12.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTGTCAAAATCTTTCAA
Rev primer name SPAC19G12.01.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAAGAGAATAAACGATAT
Amino acid length 719
Molecular weight 82.6
Isoelectric point (calc.) 5.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) SPB?; nuclear dots; nucleus>cytosol; periphery at site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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