| Gene name |
SPBC211.02c |
| Gene ID |
46/H05 |
| Gene synonyms/obsolete |
cwf3; syf1 |
| Gene product |
similarity to
drosophilla crooked neck protein; nineteen complex (Ntc)
(putative); involved in mRNA splicing; complexed with
Cdc5p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2431 |
| ORF length (spliced) |
2373 |
| Entry clone length |
2431 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC211.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAGATGTTATTCCACT |
| Rev primer name |
SPBC211.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTTTTCCTCACCAGTAATT |
| Amino acid length |
790 |
| Molecular weight |
92.6 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYDRVFELKI |
| Localization (YFP) |
SPB; nuclear dots;
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |