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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC29B12.10c
Gene ID 46/H11
Gene synonyms/obsolete
Gene product OPT family oligopeptide transporter; involved in pheromone response; involved in mating-type determination
Entry clone Cloned
ORF length (unspliced) 2556
ORF length (spliced)
Entry clone length 2556
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC29B12.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACAGCTCGCAATTCTGC
Rev primer name SPAC29B12.10.Rv
Rev primer SEQ AGAAAGCTGGGTACCAATTGGTGTAACCAAAG
Amino acid length 851
Molecular weight 96.3
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain 13
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFPLGKLLNL/LFIVVFGLTI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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