Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPJ760.02c
Gene ID 47/A08
Gene synonyms/obsolete app1
Gene product actin-binding protein; actin cortical patch component; involved in endocytosis; cofilin/tropomyosin N-terminal domain; src (SH3) homology domain; divergent repeat containing consensus PVVPE[VA]PSVPQPP[AV][AV] (10)
Entry clone Cloned
ORF length (unspliced) 2639
ORF length (spliced) 2574
Entry clone length 2639
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAPJ760.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCATTTCAACTAGATAC
Rev primer name SPAPJ760.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAATTTCCTCTACATAATTT
Amino acid length 857
Molecular weight 91.1
Isoelectric point (calc.) 4.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots at cell tip and site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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