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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC25H1.07
Gene ID 47/A10
Gene synonyms/obsolete
Gene product conserved hypothetical; conserved protein
Entry clone Cloned
ORF length (unspliced) 2658
ORF length (spliced)
Entry clone length 2658
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC25H1.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTACGGCCTCTGTGGCC
Rev primer name SPAC25H1.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATTGTACCATTTGGTATTA
Amino acid length 885
Molecular weight 100.9
Isoelectric point (calc.) 4.9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRPLWPLFL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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