| Gene name |
SPAC9G1.06c |
| Gene ID |
47/B08 |
| Gene synonyms/obsolete |
cyk3 |
| Gene product |
src (SH3) homology
domain; involved in cytokinesis; non-essential |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
2744 |
| ORF length (spliced) |
2661 |
| Entry clone length |
2744 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC9G1.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCATTCCTAAACAACT |
| Rev primer name |
SPAC9G1.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACCGCCTGCCAGGTTGCA |
| Amino acid length |
886 |
| Molecular weight |
98.2 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNSLGSSLSL/LVKEMLQALDL/LNDLEVLQI |
| Localization (YFP) |
periphery at cell tip
and site of septum formation |
| Comments for localization |
whole periphery by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |