Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPCC4B3.15
Gene ID 47/B12
Gene synonyms/obsolete dmf1; mid1
Gene product involved in cytokinesis (required); involved in septation (required); expression peaks at M-G1 phase; regulated by PBF transcription complex; involved in contractile ring positioning; non-essential; pleckstrin homology domain; no apparent orthologs
Entry clone Cloned
ORF length (unspliced) 2763
ORF length (spliced)
Entry clone length 2763
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC4B3.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAGAGCAAGAGTTCTC
Rev primer name SPCC4B3.15.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCCATAAAATTCACGTAT
Amino acid length 920
Molecular weight 102.3
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear envelope; periphery at mid region of cells (site of septum formation)
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.