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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC21D10.06c
Gene ID 47/C09
Gene synonyms/obsolete
Gene product Sp specific families; glycoprotein; serine/threonine-rich; possibly Sp specific
Entry clone Cloned
ORF length (unspliced) 2847
ORF length (spliced)
Entry clone length 2847
No. of intron 0
Sequence status Finished
Sequence results 2049T:A / 2053A:G
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC21D10.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATTCATACGCAATTTT
Rev primer name SPBC21D10.06.Rv
Rev primer SEQ AGAAAGCTGGGTAATATTCCAAAGCTCTAAGA
Amino acid length 948
Molecular weight 98.6
Isoelectric point (calc.) 3.9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFFSFERLLTL/LYPMELLTL
Localization (YFP) ambiguous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

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