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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC582.05c
Gene ID 47/C11
Gene synonyms/obsolete brc1
Gene product BRCT domain; involved in chromosome stability (required); involved in DNA repair checkpoint; non-essential
Entry clone Cloned
ORF length (unspliced) 2854
ORF length (spliced) 2637
Entry clone length 2854
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC582.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAAAAATAAAACTTTT
Rev primer name SPBC582.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAACAGCTCTTGCAAATTCA
Amino acid length 878
Molecular weight 98.4
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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