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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC553.03
Gene ID 47/D01
Gene synonyms/obsolete
Gene product AAA family ATPase; involved in peroxisome organization and biogenesis
Entry clone Cloned
ORF length (unspliced) 2859
ORF length (spliced) 2814
Entry clone length 2859
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC553.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGGTGTATTGTTTCGTA
Rev primer name SPCC553.03.Rv
Rev primer SEQ AGAAAGCTGGGTAGATTTGTCTAGTCTTACTT
Amino acid length 937
Molecular weight 105.9
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVNVPELLL/LKDFVPLQL
Localization (YFP) cytoplasmic dots; cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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