Gene name |
SPCC553.03 |
Gene ID |
47/D01 |
Gene synonyms/obsolete |
|
Gene product |
AAA family ATPase;
involved in peroxisome organization and biogenesis |
Entry clone |
Cloned |
ORF length (unspliced) |
2859 |
ORF length (spliced) |
2814 |
Entry clone length |
2859 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC553.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGGTGTATTGTTTCGTA |
Rev primer name |
SPCC553.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGATTTGTCTAGTCTTACTT |
Amino acid length |
937 |
Molecular weight |
105.9 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVNVPELLL/LKDFVPLQL |
Localization (YFP) |
cytoplasmic dots;
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |