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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC70.01
Gene ID 47/D07
Gene synonyms/obsolete SPCC1494.10
Gene product transcriptional regulator; similar to N. crassa Som1; similar to Sp SPBC1289.10c; LisH domain (SMART); single-stranded DNA binding protein (SSDP) (inferred from context)
Entry clone Cloned
ORF length (unspliced) 2895
ORF length (spliced)
Entry clone length 2895
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC70.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTGATTTCATGGACAT
Rev primer name SPCC70.01.Rv
Rev primer SEQ AGAAAGCTGGGTAATAATAAACAGATGCCGAA
Amino acid length 964
Molecular weight 104.5
Isoelectric point (calc.) 7.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery at site of septum formation, contractile ring; cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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