| Gene name |
SPAC13F5.01c |
| Gene ID |
47/D11 |
| Gene synonyms/obsolete |
msh1;
SPAC23C11.18c |
| Gene product |
MutS family; ATP
binding activity; involved in DNA repair; involved in mismatch
repair |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
2911 |
| ORF length (spliced) |
2826 |
| Entry clone length |
2911 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC13F5.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCGACATGGAGGTATAT |
| Rev primer name |
SPAC13F5.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTCATGGAAGTCGGTTTA |
| Amino acid length |
941 |
| Molecular weight |
106.9 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LANALDELDI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |