| Gene name |
SPBC947.04 |
| Gene ID |
47/E03 |
| Gene synonyms/obsolete |
|
| Gene product |
Sp specific families;
glycoprotein; serine/threonine repeat; similar to Sp
SPBC1289.15 and SPBC21D10.06 and SPCC188.09C and SPAP11E10.02C
and SPBC21D10.06C and SPBC1348.08C and SPAC977.07C; possibly
Sp specific |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2922 |
| ORF length (spliced) |
|
| Entry clone length |
2922 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
#check |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC947.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCCTGTTTCCCCAAAT |
| Rev primer name |
SPBC947.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAAGGCACAGCCTTAAAG |
| Amino acid length |
973 |
| Molecular weight |
100.8 |
| Isoelectric point (calc.) |
4.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |