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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP8B7.09c
Gene ID 47/E08
Gene synonyms/obsolete
Gene product exportin t; pre-tRNA nucleus export receptor; involved in tRNA-nucleus export; involved in tRNA splicing
Entry clone Cloned
ORF length (unspliced) 2937
ORF length (spliced)
Entry clone length 2937
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBP8B7.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGGCCCAGGATGTCGA
Rev primer name SPBP8B7.09.Rv
Rev primer SEQ AGAAAGCTGGGTATACATTACCACTTTTTAAT
Amino acid length 978
Molecular weight 110.1
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLTLLFLQL/LEKLNLLNI/LAMLGDLLNI
Localization (YFP) nuclear envelope; nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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