Gene name |
SPBP8B7.09c |
Gene ID |
47/E08 |
Gene synonyms/obsolete |
|
Gene product |
exportin t; pre-tRNA
nucleus export receptor; involved in tRNA-nucleus export;
involved in tRNA splicing |
Entry clone |
Cloned |
ORF length (unspliced) |
2937 |
ORF length (spliced) |
|
Entry clone length |
2937 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBP8B7.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGCCCAGGATGTCGA |
Rev primer name |
SPBP8B7.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACATTACCACTTTTTAAT |
Amino acid length |
978 |
Molecular weight |
110.1 |
Isoelectric point (calc.) |
4.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLTLLFLQL/LEKLNLLNI/LAMLGDLLNI |
Localization (YFP) |
nuclear envelope;
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |