Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC336.03
Gene ID 47/F03
Gene synonyms/obsolete efc25
Gene product exchange factor Cdc25p-like; guanine nucleotide exchange factor; GEF for Ras1-Scd1 pathway; deletion can worsen the growth defect of moe1; deletion synthetically lethal with tea1 deletion
Entry clone Cloned
ORF length (unspliced) 2964
ORF length (spliced)
Entry clone length 2964
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC336.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGAAGACCAAATTTGGA
Rev primer name SPBC336.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAAGAACGAGGTTCAAGT
Amino acid length 987
Molecular weight 112.7
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LNDRVINLFL/LSYLFDKSLSL
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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