Gene name |
SPAC30D11.01c |
Gene ID |
47/F07 |
Gene synonyms/obsolete |
SPAC56F8.01 |
Gene product |
alpha-glucosidase;
glycosyl hydrolase family 31; similar to Sp agl1 and
SPAC922.02c |
Entry clone |
Cloned |
ORF length (unspliced) |
2982 |
ORF length (spliced) |
|
Entry clone length |
2982 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC30D11.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGCTGTTCAAGTTTAA |
Rev primer name |
SPAC30D11.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGACCATGTTACAGTCCAG |
Amino acid length |
993 |
Molecular weight |
111 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |