Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC146.09c
Gene ID 47/G02
Gene synonyms/obsolete
Gene product L-amino acid oxidase; SWIRM domain; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 3003
ORF length (spliced)
Entry clone length 3003
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC146.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGGATTTGTCTTCGAA
Rev primer name SPBC146.09.Rv
Rev primer SEQ AGAAAGCTGGGTATGAGAAATTGTCAGGGAAT
Amino acid length 1000
Molecular weight 112.8
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYVSFNEALGI/LLHLTVLDL
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica; DeltaVision

Image information
YFP 4 images) See all images

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