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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22F3.03c
Gene ID 47/G07
Gene synonyms/obsolete
Gene product DEAD/DEAH box helicase; SNF2 family; helicase C-terminal domain; involved in DNA repair
Entry clone Cloned
ORF length (unspliced) 3040
ORF length (spliced) 2307
Entry clone length 3040
No. of intron 14
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC22F3.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAACGAAGAGCTACTTT
Rev primer name SPAC22F3.03.Rv
Rev primer SEQ AGAAAGCTGGGTATTCGAACTTTTTATGCATT
Amino acid length 768
Molecular weight 87.8
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LELIELFLSI
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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