| Gene name |
SPAC8F11.03 |
| Gene ID |
47/G08 |
| Gene synonyms/obsolete |
swi4 |
| Gene product |
MutS family; involved
in mating-type determination |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3050 |
| ORF length (spliced) |
3015 |
| Entry clone length |
3050 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC8F11.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAGGAATGAGTTATAA |
| Rev primer name |
SPAC8F11.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGATTTCTTCGAAAGCGGTA |
| Amino acid length |
1004 |
| Molecular weight |
114.6 |
| Isoelectric point (calc.) |
7.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
71/72 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear dots;
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |