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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC23E6.02
Gene ID 47/H05
Gene synonyms/obsolete
Gene product zinc finger protein; zf-C3HC4 type (RING finger); ubiquitin ligase (E3); SNF2 family; DEAD/DEAH box helicase; helicase C-terminal domain; involved in DNA repair; similar to Sp SPAC17A2.12
Entry clone Cloned
ORF length (unspliced) 3123
ORF length (spliced)
Entry clone length 3123
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC23E6.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGAAATAATACAGCTTT
Rev primer name SPBC23E6.02.Rv
Rev primer SEQ AGAAAGCTGGGTATCGTGATGACATTCCAAAT
Amino acid length 1040
Molecular weight 118.3
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LGKTVQALAL/LCMDVVAELLI
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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