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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC11E10.08
Gene ID 47/H06
Gene synonyms/obsolete rik1
Gene product involved in chromatin silencing; involved in chromatin silencing at silent mating type cassettes (sensu Fungi) (required); similar to Sp SPAC17H9.10C (paralog)
Entry clone Cloned
ORF length (unspliced) 3123
ORF length (spliced)
Entry clone length 3123
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC11E10.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTTATGTGTTCATTC
Rev primer name SPCC11E10.08.Rv
Rev primer SEQ AGAAAGCTGGGTAACATAAACTTTGTATTTCC
Amino acid length 1040
Molecular weight 118.1
Isoelectric point (calc.) 6.5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVLLQALKI/LLVFESLFI/LGPIHDLLVL/LIYFQHALKL/LGGLKFLQL/LVPVNGRLIL
Localization (YFP) SPB?; nucleus
Comments for localization nuclear dots by over expression; cytoplasmic dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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