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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3C7.08c
Gene ID 47/H10
Gene synonyms/obsolete elf1
Gene product AAA family ATPase; involved in mRNA export; non-essential; interacts genetically with rae1; interacts genetically with mex67; non-transporter with ATP binding cassette
Entry clone Cloned
ORF length (unspliced) 3174
ORF length (spliced)
Entry clone length 3174
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC3C7.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACATCCTCTGTGTTAAT
Rev primer name SPAC3C7.08.Rv
Rev primer SEQ AGAAAGCTGGGTATTCTTCATCATCAGAGAAG
Amino acid length 1057
Molecular weight 116.7
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLSRLKRLVL
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: cytosol=nucleus
Microscope used for observation Leica

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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