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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC2G2.08
Gene ID 47/H11
Gene synonyms/obsolete ade9
Gene product C-1-tetrahydrofolate synthase; methylenetetrahydrofolate dehydrogenase; methylenetetrahydrofolate cyclohydrolase; formyltetrahydrofolate synthetase; involved in purine biosynthesis
Entry clone Cloned
ORF length (unspliced) 3177
ORF length (spliced) 2910
Entry clone length 3177
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC2G2.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTTCGTTTAATCAATT
Rev primer name SPBC2G2.08.Rv
Rev primer SEQ AGAAAGCTGGGTATGACAGCCCAACAATTTCT
Amino acid length 969
Molecular weight 105.4
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVTTVKALKL
Localization (YFP) mitochondrion
Comments for localization aggregates
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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