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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1223.06
Gene ID 48/C11
Gene synonyms/obsolete tea1
Gene product tip elongation aberrant microtubule-associated protein; involved in cell polarity; involved in cellular elongation; kelch repeat protein; target of Shk1p; phosphorylated by Shk1p; involved in cytokinesis; involved in microtubule based movement; involved in cell-end anchoring (required); similar to Sp tea3
Entry clone Cloned
ORF length (unspliced) 3444
ORF length (spliced)
Entry clone length 3444
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC1223.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTTTTTATTTAAAAG
Rev primer name SPCC1223.06.Rv
Rev primer SEQ AGAAAGCTGGGTAATTTTCGTTGTCATGGACT
Amino acid length 1147
Molecular weight 127.4
Isoelectric point (calc.) 6.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots at cell tip and site of septum formation; microtubules
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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