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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC30D11.04c
Gene ID 48/D05
Gene synonyms/obsolete nup124
Gene product nuclear pore complex
Entry clone Cloned
ORF length (unspliced) 3523
ORF length (spliced) 3480
Entry clone length 3523
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC30D11.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTCCTGTTTCAAAAAA
Rev primer name SPAC30D11.04.Rv
Rev primer SEQ AGAAAGCTGGGTAACGTTTTCTTCGACTTCGG
Amino acid length 1159
Molecular weight 123.9
Isoelectric point (calc.) 10.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear envelope
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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