| Gene name |
SPCC5E4.06 |
| Gene ID |
48/D06 |
| Gene synonyms/obsolete |
rad18 |
| Gene product |
involved in DNA
repair; involved in DNA damage response (required); SMC6
complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3529 |
| ORF length (spliced) |
3423 |
| Entry clone length |
3529 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC5E4.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTACAGAGCTTACTAA |
| Rev primer name |
SPCC5E4.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGGCGCCGTAGAGGGAGGA |
| Amino acid length |
1140 |
| Molecular weight |
130.9 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHTLINHLGI |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |