| Gene name |
SPAC10F6.02c |
| Gene ID |
48/D08 |
| Gene synonyms/obsolete |
prp22 |
| Gene product |
ATP-dependent RNA
helicase; involved in mRNA splicing; complexed with
Cdc5p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3560 |
| ORF length (spliced) |
3507 |
| Entry clone length |
3560 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC10F6.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACGATCTGAAAGAATT |
| Rev primer name |
SPAC10F6.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGACCTCCCTTTCGTTGC |
| Amino acid length |
1168 |
| Molecular weight |
131.4 |
| Isoelectric point (calc.) |
7.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKELEYLSL/LGDSIPELVI |
| Localization (YFP) |
nuclear dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |