Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31G5.19
Gene ID 48/D10
Gene synonyms/obsolete
Gene product TAT-binding protein homolog; AAA family ATPase; bromodomain protein; similar to Sp SPBP22H7.05C (paralog)
Entry clone Cloned
ORF length (unspliced) 3573
ORF length (spliced)
Entry clone length 3573
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC31G5.19.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGGAAGAAGCAAGTGA
Rev primer name SPAC31G5.19.Rv
Rev primer SEQ AGAAAGCTGGGTATTGTAGTGCATTAATCATA
Amino acid length 1190
Molecular weight 135.3
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKEMVMLPL/LETFETLRI/LTPLKQLLI
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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