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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP35G2.06c
Gene ID 48/D11
Gene synonyms/obsolete
Gene product nucleoporin; nuclear pore complex (putative)
Entry clone Cloned
ORF length (unspliced) 3584
ORF length (spliced) 3429
Entry clone length 3584
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBP35G2.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTATTGTACATCGACG
Rev primer name SPBP35G2.06.Rv
Rev primer SEQ AGAAAGCTGGGTATTGTTTTGTTGCTACGTCC
Amino acid length 1142
Molecular weight 131.4
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVAFKYLHL/LKKCLNINLLL
Localization (YFP) nuclear envelope; cytosol; periphery at site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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