Gene name |
SPBP35G2.06c |
Gene ID |
48/D11 |
Gene synonyms/obsolete |
|
Gene product |
nucleoporin; nuclear
pore complex (putative) |
Entry clone |
Cloned |
ORF length (unspliced) |
3584 |
ORF length (spliced) |
3429 |
Entry clone length |
3584 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBP35G2.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTATTGTACATCGACG |
Rev primer name |
SPBP35G2.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTGTTTTGTTGCTACGTCC |
Amino acid length |
1142 |
Molecular weight |
131.4 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVAFKYLHL/LKKCLNINLLL |
Localization (YFP) |
nuclear envelope;
cytosol; periphery at site of septum formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |