Gene name |
SPAC1565.07c |
Gene ID |
48/E07 |
Gene synonyms/obsolete |
|
Gene product |
TATA binding protein
interacting protein; involved in transcriptional regulation;
no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
3701 |
ORF length (spliced) |
3663 |
Entry clone length |
3701 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1565.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGGAGGGAATTTTATT |
Rev primer name |
SPAC1565.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTTTTAATTGTAGTTTTG |
Amino acid length |
1220 |
Molecular weight |
137.8 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCVVCDSLEI/LTQKLLEVLGL/LSRLEYLPI/LSIVSELLNL/LLEHILGLLI/LKCLLIIPLSI/LSVFRFSLTL |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |