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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1486.05
Gene ID 48/H04
Gene synonyms/obsolete nup189
Gene product nucleoporin; nuclear pore complex; involved in nuclear import; involved in nuclear export; involved in nuclear export of the small ribosomal subunit; interacts physically with Ned1p (2-hybrid); essential
Entry clone Cloned in 2006 trial
ORF length (unspliced) 5423
ORF length (spliced) 5337
Entry clone length 5423
No. of intron 1
Sequence status Partially sequenced
Sequence results 100% match in both ends
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1486.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTGGACAAAACAATTC
Rev primer name SPAC1486.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAAATTGCACAGATATATTT
Amino acid length 1778
Molecular weight 189.5
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LNYRVVWQLAI/LWSLFVLLHL/LTDAICNLPL
Localization (YFP) no expression clone
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation

Image information
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