| Gene name |
SPAPB2B4.05 |
| Gene ID |
49/A08 |
| Gene synonyms/obsolete |
vma5 |
| Gene product |
vacuolar ATP synthase
(subunit C); V-type ATPase (C subunit); vacuolar proton pump
component; V1 sector |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1625 |
| ORF length (spliced) |
1185 |
| Entry clone length |
1625 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
482C:T / 1248A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAPB2B4.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAAAAGTGATTTCTG |
| Rev primer name |
SPAPB2B4.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATAATTAAACACCTTTGAA |
| Amino acid length |
394 |
| Molecular weight |
44.3 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDESMADLNL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
Leica |