| Gene name |
SPBC776.15c |
| Gene ID |
49/B05 |
| Gene synonyms/obsolete |
|
| Gene product |
dihydrolipoamide
S-succinyltransferase (e2 component of oxoglutarate
dehydrogenase complex); involved in 2-oxoglutarate metabolism;
involved in tricarboxylic acid cycle; dihydrolipoamide
S-succinyltransferase activity |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1711 |
| ORF length (spliced) |
1359 |
| Entry clone length |
1711 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC776.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTTCTTACGGCAATGG |
| Rev primer name |
SPBC776.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACTAATAGCATTTTTGCA |
| Amino acid length |
452 |
| Molecular weight |
48.9 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
aggregated
mitochondria by over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |