| Gene name |
SPAC977.16c |
| Gene ID |
49/C03 |
| Gene synonyms/obsolete |
dak2 |
| Gene product |
dihydroxyacetone
kinase; induced by glycerol (WB); repressed by glucose
(WB) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1776 |
| ORF length (spliced) |
|
| Entry clone length |
1776 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
6T:G / 745T:C /
1303T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC977.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGTAAAACAATTTGT |
| Rev primer name |
SPAC977.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGTTCGCTTGCCTCAACA |
| Amino acid length |
591 |
| Molecular weight |
62.1 |
| Isoelectric point (calc.) |
5.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
bright dots by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |