Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC11B10.01
Gene ID 49/C05
Gene synonyms/obsolete SPBC32H8.14; pi010; c11B10.01
Gene product glycosyl transferase family 1; involved in oligosaccharide-PP-dolichol assembly; alpha-1, 3-mannosyltransferase; human disease associated (CDG-Ii)
Entry clone Cloned
ORF length (unspliced) 1787
ORF length (spliced) 1536
Entry clone length 1787
No. of intron 3
Sequence status Finished
Sequence results 250G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC11B10.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCACAAGAAAATTCGGT
Rev primer name SPBC11B10.01.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGATTCGTTAGCTTTGCA
Amino acid length 511
Molecular weight 57.8
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain 4
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LPSSIFGRLSI/LSTCVPFLLL
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Leica, Confocal

Image information
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