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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC12B10.12c
Gene ID 49/C12
Gene synonyms/obsolete rhp41; rhp4a
Gene product involved in DNA repair; involved in nucleotide-excision repair; XP-C family; repairosome; similar to Sp rhp42 (paralog)
Entry clone Cloned#/2004.10 & Cloned#/ 3' FS
ORF length (unspliced) 1917
ORF length (spliced)
Entry clone length 1917
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC12B10.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGAGTGACGAAGATAA
Rev primer name SPAC12B10.12.Rv
Rev primer SEQ AGAAAGCTGGGTAACCGTATTCTTCGAATACA
Amino acid length 638
Molecular weight 73.5
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 266
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LWKRLITGLRI
Localization (YFP) nucleus; spindle microtubules
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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