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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP22H7.01c
Gene ID 49/D06
Gene synonyms/obsolete SPBC691.05c; pi030
Gene product putative ion channel protein
Entry clone Cloned
ORF length (unspliced) 2136
ORF length (spliced) 2007
Entry clone length 2136
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP22H7.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATGAGAAATTGCAAAG
Rev primer name SPBP22H7.01.Rv
Rev primer SEQ AGAAAGCTGGGTACTTTGTTTTCTTGTTTTCA
Amino acid length 668
Molecular weight 76.6
Isoelectric point (calc.) 8.5
Signal SEQ
No. of transmembrane domain 9
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSSLLRELQL/LPAVVFQVLTL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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