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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB1A10.09
Gene ID 49/D11
Gene synonyms/obsolete
Gene product microtubule-associated protein; involved in spindle elongation (anaphase); similar to human prc1, protein regulating cytokinesis
Entry clone Cloned
ORF length (unspliced) 2243
ORF length (spliced) 2196
Entry clone length 2243
No. of intron 1
Sequence status Finished
Sequence results 1203T:C / 1547T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAPB1A10.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAAACAGTAATGATGGA
Rev primer name SPAPB1A10.09.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGCCTTCTTCTCCCCAT
Amino acid length 731
Molecular weight 83.1
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LPKVQSLLI
Localization (YFP) microtubules
Comments for localization only spindle microtubules?
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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